Hypothesis
Suppose we have four test tube each test tube is filled with solution extracted from different sources. Test tube 1 contain solution extracted from sugar cane, Test tube 2 contain solution extracted from green leaves, Test tube 3 contain solution extracted from seeds, and Test tube 4 contain egg white an albumin protein.
objectives
To find out which solution will react with biuret solution
.
Materials and method
To prepare biuret solution, mix potassium sodium tartarate with cupric sulfate and sodium hydroxide. A blue solution will be formed.
Experiment
Pour a small amount of biuret solution in each test tube and heat them until precipitation is formed.
Results
After analyzing the experiment, it was found that no color changed in first three test tubes except the fourth one in which the color was changed from blue to pink-purple.
Discussion
It was deduced from results that appearance of pink-purple color indicate the presence of peptide bonds (proteins)
Conclusion
Biuret test is used for the confirmation of proteins in any food sample.
