Question

you fill a cuvette with a solution of enzyme, substrate, and dye that changes color as the reaction occurs. You put in a spectrophotometer and record the absorbance at several time points. The absorbance increases at first, then starts to level off, staying almost exactly the same at each time point. Why did the absorbance increase at the beginning and why did it level off over time?​

Answer 1

Answer:

 the change in absorbance reflects the reaction rate. As the reaction completes, its rate slows, so the absorbance levels off.

Explanation:

A spectrophotometer measures the amount of light of a particular color passing through the sample. The changing color of the dye will change the value read by the spectrophotometer..

Apparently, the absorbance increased as the reaction progressed. When the reaction was almost complete, the absorbance increase slowed or stopped.