When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
stomata are tiny pores present on a surface of leave which helps in exchange of gases
Aqueduct carries water over a long distance.
Answer:
The word for the blank spot is Recessive.
Explanation:
Answer:
During <u>fertilization</u>, chromosomes from haploid gametes combine, and a <u>diploid</u> zygote is formed.
Explanation:
Fertilization is the union of the male gamete(haploid chromosomes) with the female gamete(haploid chromosome) to form a diploid cell called zygote (with diploid chromosomes).
