The lines in this excerpt from the poem <em>A Grain of Sand</em> by Frances Ellen Watkins Harper that indicate its subject for me are:
(Do you see this grain of sand)
(Oh how little seems the span)
In this poem, the narrator is comparing a grain of sand with the significance of human life. She uses the grain of sand to show how small can a part of the beach be, how it is "<em>lying loosely</em>" in her hand and how us, individuals, are like a grain of sand in the Universe, "<em>how little seems the span</em>".
The idea of the whole poem is to show how small and insignificant can a part of everything seem to be.