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docker41 [41]
4 years ago
5

During a pulse-chase experiment, photographic emulsions were prepared at different times during the chase, and radioactive spots

were detected at the following times and locations: 5 minutes: rough ER; 10 minutes: Golgi apparatus; 40 minutes: endosomes; 70 minutes: lysosomes; 140 minutes: lysosomes. Which of the following conclusions can be drawn from these results
Biology
1 answer:
NikAS [45]4 years ago
5 0

Answer:

Pulse chase experiment was performed to find the secretory pathway of proteins inside the cell. The protein sorting and pathway of protein can easily be studied by the pulse chase experiment.

The molecule is labelled with radioactive component and its pathway is observed during the pulse chase experiment. The molecule reside  5 minutes in rough ER. After this 40 minutes in glogi apparatus. From the golgi apparatus the molecules moves towards the endosomes and resides for 70 minutes. The molecules then transferred to lysosomes in 140 minutes. This chasing explains the pathway of the molecule.

The pathway can be drawn is ER→golgi→endosome→lysosome.

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An enzyme that follows simple michaelis–menten kinetics has an initial reaction velocity of 10 μmol⋅min−1 when the substrate con
svet-max [94.6K]

The Vmax of the enzyme is 12 μmol⋅min−1

We use Michaelis-menten equation,  which is given by;

V=\frac{Vmax(S)}{Km+(S)} \\

Where v is velocity of reaction

Vmax = maximum rate achieved by the system

[S] = concentration of a substrate

K_{m}= Michaelis constant

Given V_{0} = 10umolmin^{-1}

[S] = 5km

Now, we calculate Vmax:

10umolmin^{-1} = \frac{Vmax*(5Km)}{Km+5Km}

Km = 1

10=\frac{5KmVmax}{6Km}

\frac{10*6}{5} =Vmax

Vmax =12 μmol⋅min−1

Learn more about Michaelis-menten kinetics here:

brainly.com/question/15180587

#SPJ4

6 0
1 year ago
Plants maintain higher levels of phytochrome at their growing tips where phytochrome plays important roles in growth responses t
Naddika [18.5K]

Explanation:

Phytochrome is a protein with kinase activity present in plant organisms, whose function is to act as a photoreceptor mainly of red light (600-700nm) and distant red light (700-800nm), thanks to its chromophore. The phytochrome depending on the type of light detected can trigger different responses in the plant, such as flowering, germination, growth as an escape response to the shadow -development of epicotyls at night and cotyledons during the day-, regulation of expression of metabolic activity during day and night (circadian rhythms).

Phytochromes were discovered in the fifties as part of an investigation on the effect of light on the germination of lettuce seeds. It was observed that the seeds that germinated in the dark did not reach 20%; On the contrary, the germination percentage was maximum when the seeds were irradiated with a pulse of red light (R). It was also found that subsequent irradiation with a pulse of distant red light (RL) nullified the inducing effect of red light, preventing germination.

Alternating irradiation with light R and RL (R, R + RL, R + RL + R, R + RL + R + RL, etc.) showed that the last color applied determined the germination of the seeds and that the light red was the stimulating factor of the process and, its inhibitor, the distant red light.

In search of an explanation of such phenomena, the existence of a pigment was proposed, which they called phytochrome, which absorbed the red light. The phytochrome in question, after absorbing red light, became a way capable of absorbing distant red radiation; form that turned to its initial condition after performing said absorption. The hypothesis found experimental support in the early sixties with the purification, from extracts of cereal seedlings, of a protein endowed with the predicted characteristics. Phytochromes are soluble proteins found in the seeds, leaves, stems, roots and other organs of the plant.

Biochemically, phytochrome is a protein with a Bilin chromophore.

6 0
3 years ago
DNA is also a ___________ ___________, which means it is composed of thousands of subunits called ________
Mars2501 [29]
I don’t know the first two but the last one the thousands of subunits are called nucleotides
6 0
3 years ago
Pls help with these three questions
Mariulka [41]
9. Normal tides would remain the same but neap and spring tide cycle would be cut in half. Meaning if there was a neap tide after every 14 days it would come 2 times faster (after every 7 days)
4 0
3 years ago
In a paragraph explain what effect very small pores and their diffusion rate would have on the workings and mechanism of a cell
Murrr4er [49]

Answer:

So if the size of the pore is small then the rate of the diffusion will be very slow as the molecules will move from high concentration to low concentration in very slow rate.

Explanation:

I hope it helps you

6 0
3 years ago
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