Someone plz help with this inquiry skill question. Thank you

Which step in the PCR program would you have to change if your fragment would have been 10 times longer and how?

bekas [8.4K]

Answer:

increasing extension time

Explanation:

The Polymerase Chain Reaction is a technique widely used in molecular biology laboratories to amplify target DNA regions. The standard steps of a PCR are as follow 1-denaturation, 2-annealing and 3-elongation/extension. These steps are repeated 15-40 times in order to exponentially amplify the linear DNA fragment. It is well known that longer extension times can be used as a strategy to increase the yield of longer PCR products. This is because the extension time depends on the synthesis rate of the DNA polymerase used in PCR technique and the length of the DNA fragment to be amplified.

7 0

3 years ago

Changes in the gene pool can occur due to various mechanisms. From 1892 to 1954, millions of individuals emigrated (moved out) f

kherson [118]

The mechanism that affected the gene pool of the immigrants that entered the United States through Ellis Island from 1892 to 1954 is B. gene flow. Gene flow is the transfer of genes or alleles from one population to another. The migration of millions of individuals from countries across the Atlantic Ocean to the United States is responsible for the changes in the gene pool of the immigrants.

6 0

3 years ago

Read 2 more answers

You should carry the microscope with the base in the palm of one hand and the other hand on the arm.

Sergeu [11.5K]

The answer is

A. True

3 0

3 years ago

Consider a cross between a plant with two flower colors, yellow and red. Yellow (Y) exhibits complete dominance over red (y). It

Aneli [31]

Answer:

1) option a is correct. (260+270)/1545

(Note about this option: In the statement, it is written as (260+270) 1545. Lacks the division symbol, / )

2) From these results one can conclude that the two genes are linked (option a)

Explanation:

To know if two genes are linked, we must observe the progeny distribution. If heterozygous individuals, whose genes assort independently, are test crossed, they produce a progeny with equal phenotypic frequencies 1:1:1:1. If we observe a different distribution, phenotypes appearing in different proportions, we can assume linked genes in the double heterozygote parent.

We might verify which are the recombinant gametes produced by the di-hybrid, and we will be able to recognize them by looking at the phenotypes with lower frequencies in the progeny. Phenotypes with the highest frequencies represent the parental gametes.

To calculate the recombination frequency, we will use the following formula: P = Recombinant number / Total of individuals.

In the present example:

Parental) YySs x yyss

Gametes) YS parental type ys, ys, ys, ys

ys parental type

Ys recombinant type

yS recombinante type

The total number of individuals in the offspring: 1545

Phenotypic class Number of offspring

500 Y-S- (parental)
515 yyss (parental)
260 Y-ss (recombinant)
270 yyS- (recombinant)

According to this information, the phenotypic frequencies of the progeny differ from the phenotypic ratio 1:1:1:1. We can assume then, that these genes are linked.

The recombination frequency is:

P = Recombinant number / Total of individuals

P = 260 + 270 / 1545

P = 530/1545

P = 0.343

The genetic distance between genes is 0.343 x 100= 34.4 MU.

6 0

3 years ago

Which of the following is true in regards to the cell membrane?

iris [78.8K]

Answer:

It is also referred to as the phospholipid bilayer or the plasma membrane, It helps cells maintain homeostasis, It is selectively permeable; that is allows some substances through but not others

Explanation:

a. I don't know how to explain other names for the cell membrane but they are it, as the cell membrane is a bilayer composed of phopholipids and it is also called a plasma membrane

b. The cell membrane has many structures on it that facilitate nutrient transport in and out of the cell (ex: protein pump). nutrients of higher concentrations would like to move through lower concentrations, and it achieves this by moving across the cell membrane in or out of the cell, maintaining homeostasis

c. Some substances such as NaOH use pumps on the cell to move in or out of it, it can't get in or out without using pumps since they are so big, so the cell can choose when the cell wants it's nutrients or if it dosen't

7 0

3 years ago