Plants are main producers in ecosystems so, primary productivity of ecosystems depends on them. Carbon is necessary for plants, but it is widely present in plants. On the other hand, calcium and sodium are not as essential. However, nitrogen is important for plants because it is necessary to make proteins. Proteins take part in many different processes such as photosynthesis (this is actually primary productivity). But, nitrogen is not available to plants, they cannot absorb it simply from the air but rely on some symbiotic bacteria which are able to.
Microtubule will form the spindle fibers that will grab the chromosome. The formation of microtubule will be start from prophase and it will have role in moving the chromosome through the metaphase and anaphase. If the substance disrupting the formation of microtubules, then spindle fibers will not be formed.
