Select all that apply. Which of the following are parts of a desert community? jack rabbit plankton beaver cactus crayfish

Design an experiment to answer the question, what is the effect of caffeine on the rate of mitosis in frog eggs? Label: hypothes

s2008m [1.1K]

Answer:

Explanation:

Null Hypothesis: Their will be no effect of the caffeine on the mitosis.

Hypothesis: Caffeine will inhibit the SHSY5Y cell division.

Dependent variable: Various concentration of the caffein compound.

Independent Variable: temperatures of the experimental condition. If their will be any effect of the temperature in mitosis then it will also affect the mitosis of control cells.

Experimental design.

Culture SHSY5Y neuronal cell line in the T-75 culture flask in CO2 incubator at 37 degree celsius. Next day, add cells from T-75 flask in the 24 well plate and allow cells to adhere to the well by incubating plate in CO2 incubator at 37 degree celsius. Add the compound caffeine at different concentration (say 1 microgram, 2 microgram, 3 microgram, 4 microgram) and incubate the plate for 24 hour. Determine the mitotic cells by following assay.

Assay to determine mitotic cell.

The reagent succinimidyl ester of carboxyfluorescein diacetate (CFSE) binds to cytoplasmic proteins. it is fluroscent reagent. And when cell divide due to mitosis, cytoplasm will also divide and hence, the protein which stained with the CFSE reagent will also goes in the daughter cell. Then, the fluorescence of the stained cells can be read on the plate reader.

Prepare the graph of the fluorescence values (Y-axis) verses different concentrations of the caffein (X-axis) and determine the slop of curve to know the rate of mitosis.<u> </u>

<u>Following test and control samples will be used for the assay.</u>

1. SHSY5Y cell line with added caffeine. It is test sample.

2. SHSY5Y cell line added with buffer. This important to know behaviour of cells under experimental conditions.

3. SHSY5Y cell line with solvent used to dissolve compound caffeine. It is important to know the toxicity or any effect of solvent on the mitosis.

5 0

2 years ago

What is secondary succession?

GuDViN [60]

Secondary succession is a process started by an event (example forest fire) that reduces an already established ecosystem

3 0

2 years ago

How is it possible that over 20 million living organisms on the planet are made up of only 5 types of nucleotide bases arranged

Tpy6a [65]

<h2>Answer</h2>

It is because of different arrangements of nucleotide bases.

<h2>Explanation</h2>

There are only five types of nucleotide basis are present in the genetic material which makes up the all the genetic information of the individuals. These nucleotides are named as Adenine, Thymine, Guanine, Cytosine and Uracil. In all the living beings on the earth, these only five nucleotides are present which decides their behaviour and functions on the earth. These five bases have features to get any arrangements according to the situation. Their different arrangement at the heredity material makes the difference in all living beings regarding their behaviour.

4 0

3 years ago

Which areas receive more sunlight as compared to the polar areas? the areas receive more sunlight as compared to the polar areas

Naddika [18.5K]

The answer is tropical areas. The tropical areas obtain more radiant energy from the Sun than they lose to space through terrestrial radiation, and vice versa for polar areas. Earth has an energy remaining in the tropics and an energy scarcity for the polar areas.

8 0

2 years ago

Which element is more important to life, water or carbon? why?

stellarik [79]

Carbon would be the answer to this because it is an element while water is a compound. In addition, carbon had 4 bonding sites which allows it to form a variety of molecules like sugars, proteins, and even DNA that are essential for life as we know it.

3 0

2 years ago