Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Vacuole is not a structure used for cellular movement
Answer:
D) Even a slight reproductive advantage will eventually lead to the elimination of the less well adapted of two competing species.
Explanation:
The competitive exclusion describes relationship between two species that compete for the limiting sources, and cannot coexist. It is enough for one species to have slightly advantage to be dominant over the other. As a consequence, other species (the weaker one) will be extincted (shift to a different ecological niche).
<span>The best place would be far away from a cliff and the worst would be right on top of a cliff or some sort of land such as a beach or directly beneath it. At anytime, gravity will cause mass wasting, in other words the rock and soil will just break apart.</span>
