Answer:
option A
Explanation:
In carrying out these experimental procedure, the same restriction enzyme that is used to cut out the gene of interest should also be the same used for the cutting of the plasmid to generate sticky ends. Then the desired gene is inserted into the cut plasmid then treatment with DNA ligase. Purified DNA ligase is utilized in gene cloning to join/ligate DNA molecules together forming a recombinant DNA. Thus, step 3 should be carried out after step 4.
Answer:
probably the last one because winds tend to travel from colder areas to hotter areas
A lit candle needs to draw oxygen from the air in order to continue burning. If you limit the amount of air available, the candle's flame eventually goes out once it uses up all the oxygen. Lit candles in smaller jar stop burning first because the oxygen trapped in the jar are fewer than the ones in bigger jars.
ANSWER:⇒ cork
People struggled to believe that some of Hooke's images were real because they were so strange and remarkable! Hooke noticed tiny boxlike spaces in cork under his microscope, which he depicted and characterized as cells. Plant cells have been discovered by him!
