Answer:
a. Release of acetylcholine from the synaptic vesicles
Explanation:
The events on neuromuscular junction:
Action potential travels through the membrane of the presynaptic cell causing the voltage-gated channels permeable to calcium ions to open. Ca2+ flow through the presynaptic membrane and increase the Ca concentration in the cell which will activate proteins attached to vesicles that contain a neurotransmitter (e.g. acetylcholine). Vesicles fuse with the membrane of the presynaptic cell, thereby release their contents into the synaptic cleft-space between the membranes of the pre- and postsynaptic cells. Neurotransmitter ACh binds to its receptors on the postsynaptic membrane and its binding causes depolarization of the target cell (muscle cell). Depolarization occurs because sodium enters the cell as a result of neurotransmitter receptor binding.
Cause living things can not directly use most of matter which they found in nature. For example carbon, all organism use carbon in complex structure, that is in compound or macromolecule(glucose,fructose,amino acid). we can not directly earn ATP from C which we found in soil/other sources.
