When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
It would be best to use an MRI to check for any torn ligaments.
Hope this helps :)
Hypotonic - because there is a higher concentration inside the cell than outside of it
A DNA molecule consists of two strands of nucleotides twisted together to form a double helix. The sugar-phosphate backbone is found on the outside of this helix and the bases are found braching towards the middle. Hydrogen bonds join the thenitrogenous bases and hold the two strands together.
Therefore your answer is sugar and phosphate.
