Apart from restriction enzymes, there are four useful nucleases that are often used in genetic engineering. These are – Bal 31 and – Exonuclease III (exonucleases), and – Deoxyribonuclease I (DNase I) and – S1-nuclease (endonucleases). If you want to know more about enzyme engineering, the link below may help https://www.creative-enzymes.com/service/Enzyme-Engineering-And-Modification_66.html