Question

A plasmid vector contains a single ecori site in the polylinker (multiple cloning site). a gene of interest is flanked by two ecori sites, one at each end. you cut the vector and the dna containing your insert with ecori, and ligate the two. how many ecori site(s) is/are present in the recombinant dna molecule and how many band(s) would you see when you run your digest of recombinant dna on a gel? (assume that there is no internal ecori site in the insert dna.)

Answer 1

U should pay attention