Answer:
<em> A nick is created between G and A in GAATTC when using Eco R1 site while a nick is generated between G and G in GGATCC when Bam H1 is used. The difference in the nicks created is the main reason you observe two bands when you digest the circular plasmid with a mixture of both</em>
Explanation:
Circular plasmid containing : 9 kb of DNA
Eco R1 and Bam H1 are known as restriction sites in DNA ( i.e. special sequences found in a DNA ) hence they can be specifically cleaved by the restriction enzymes enabling the insertion of a gene. that is why you will get a same result when you digest the Plasmid with either Eco R1 or Bam H1
<em>but A nick is created between G and A in GAATTC when using Eco R1 site while a nick is generated between G and G in GGATCC when Bam H1 is used. The difference in the nicks created is the main reason you observe two bands when you digest the circular plasmid with a mixture of both</em>
Answer:
Explanation:
there is nothing on the slide
they are useful in agriculture because of their genetic diversity
Answer: heres your awnser
Explanation:
Removing wolves from the park affected much of Yellowstone because wolves are top predators and arguably keystone species. ... Wolves feed on elk, and without the wolves, the elk population exploded. The elk fed on young aspen trees, so the park had very few young aspen trees