The triose phosphate isomerase (TPI) is the glycolitic enzyme that catalyzes a reaction with an enediolate intermediate. It <span>plays an important role in efficient production of energy and is essential in </span>glycolysis. This<span> </span>enzyme catalyzes<span> the reversible interconversion of the </span>triose<span>phosphate </span>isomers dihydroxyacetone phosphate<span> and D-</span>glyceraldehyde 3-phosphate<span>.</span>
Answer: CFU/mL = 14200 CFU/mL
Explanation:
Microorganisms are very small and difficult to count directly. Instead, a laboratory technique is used to count CFUs (Colony Formation Units). This involves making dilutions of a microbial suspension and spreading it on thin layers of agar containing nutrients for growth in Petri dishes also called agar plates. After incubation under ideal conditions, some plates are completely covered with microbial growth; some have little or no growth; and some are covered with individual isolated "spots", which are microbial colonies. Since a colony may consist of a single organism or a group of microbes, the dots represent a CFU.
To calculate the viable count of the stock culture, you have to count the number of colonies, multiply by the dilution and the result will be the plate count in CFU.
So, the equation is:
CFU/mL = Number of colonies / (Volume trasferred to plate x dilution blank)
100 microliters = 0.1 mililiter
1/10 = 0.1 (dilution factor)
Replacing the information from the question:
CFU/mL = 142 / (0.1 mL x 0.1)
CFU/mL = 14200 CFU/mL
Answer:
An atom is the smallest particle in a chemical element that holds its chemical properties. Although in the past it was thought impossible to break an atom, within modern chemistry we know that an atom is composed of subatomic particles, which compose the atomic model:
1) electrons, which have a negative charge, such a small size that it is immeasurable, and a mass much smaller than the other two subatomic particles; 2) protons, which have a positive charge; and 3) neutrons, which have no charge.
The protons and neutrons form a dense and massive atomic nucleus, which is called multipons nuclei. The electrons form a cloud of electrons that surround the nucleus.
Clonal selection identifies a __ lymphocyte that needs to be cloned for production of a specific antibody.
is B.