As temperature increases, double stranded dna breaks apart in two separate stages. why might this be?

2 answers:

5 0

The double-stranded DNA (Deoxyribonucleic acid) breaks into two separate strands when the temperature is increased, such as in the denaturation step of the PCR (Polymerase Chain Reaction), because the increase in the temperature makes the hydrogen bonds between the nitrogenous bases to break. The double hydrogen bond between the adenine and thymine, and the triple hydrogen bond between the guanine and cytosine breaks apart when heated causing the two DNA strands to fall apart.

Hence, the answer is the breaking of the hydrogen bond between the nitrogenous bases.

stira [4]4 years ago

3 0

<h2>Answer:</h2>

As the temperature increase the covelent bonds between the bases of two strands breaks making two single strands of DNA.

<h3>Explanation:</h3>

Two strands of DNA are attached to each other by the hydrogen bonds between the nitrogenous strands of two bases.
There is double hydrogen bond between the adenine and thymine, and the triple hydrogen bond between the guanine and cytosine bases.
As the temperature increase kinetic energy of covelent bond pair increase resulting in bond breakage.

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