The reaction of the American colonists to the Proclamation of 1763 was that they angrily ignored it.
Britain gained new and vast portions of land after the French and Indian War and the Seven Years War. That is why British King George III issued the Royal Proclamation of 1763. It prohibited colonist to cross the Appalachian Mountains and moved West to the new territories. Of course, this did not like the American colonists and angrily ignored it. There was no real way for the British to control access into the western territories.
Yes, studying how mayfly population affects the number of trout in a stream is ecology. Ecology seeks to unveil how different organism relate with one another as well as their environment. Ecology is basically the study of distribution,abundance and relation of organisms and their interaction with their environment.