Answer:
A vaccum should be used for the control group chamber.
Explanation:
In an experiment, a control is an experiment or observation designed to minimize the effects of variables other than the independent variable. The introduction of a control increases the reliability of the results; often through a comparison between the control measurements and the other measurements.
A vaccum according to this experiment is a chamber that will have no pressure within it. This will give a benchmark of the effect of pressure in this experiment.
The allele which has light color appears to be altering transcription and that is why the corresponding mRNA is absent in zebrafish which is homozygous. The protein we can say were absent because mRNA which is being required for protein synthesis it was not present.
If the allele had altered translation, mRNA which is functional would have been present but in the same case, the protein will still be absent.
The first one should be mM
