Answer: Issac became stubborn and defensive because of the fierce nature of siblings and commanding nature of his second father and mother. Due to the stubbornness of his family member he had to leave his study in early childhood and engage in farming which he left ultimately to pursue higher studies.
Explanation:
Answer: Spiritual education
Explanation:
The purpose of providing the spiritual education is to provide the divine potential in individuals. It helps in recognizing the religion as an important expression. It helps in understanding the existence of good with us and learn values, beliefs, and practices which once said and followed by god. It helps in transforming character of a person and prevents in conduction of evil practices. It helps in engagement in intellectual and spiritual enrichment.
The unique property of an echinoderm within higher invertebrates is their radial symmetry. These echinoderms include the famous starfish, sea urchin, sand dollars, sea cucumbers, and sea lilies. They are found at the ocean depth, form the intertidal zone to the abyssal zone.
Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
