1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
inysia [295]
3 years ago
15

A bacterial sample has a cell density of 1.85 x106 CFU/mL. What plate dilution should yield a countable plate? Which two dilutio

n tubes could produce this plate dilution? How
Biology
1 answer:
insens350 [35]3 years ago
7 0

Answer:

Explanation:

Bacterial count in stock- 1.85x10^6 cfu/ml

Dilution methods

Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.

Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.

Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.

Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml

So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.

If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.

You might be interested in
If we put a plant and animal cell into a solution that contains no solutes at all , the solution is______
frosja888 [35]
The answer is isotonic
3 0
3 years ago
Read 2 more answers
Convert 0.0701 kg to milligrams
aliina [53]
To convert 0.0701 kilograms to milligrams, we must first figure out how many milligrams are in 1 kilogram.

1 kg (kilograms) = 1,000,000 mg (milligrams).

Since we have 0.0701 kg, we need to multiply 1,000,000 by 0.0701.
1,000,000 x 0.0701 = 70,100.

There are 70,100 mg in 0.0701 kg.

I hope this helps!
3 0
3 years ago
How are neurotransmitters and hormones similar and how are they different?
ratelena [41]

Answer:

Both neurotransmitters and hormones influence our thoughts and motivations, as well as our ability to learn and concentrate. However, neurotransmitters are short-lived while hormones act for longer periods of time.

5 0
3 years ago
Which characteristic of water allows for a spider to glide across the top of the water due to the strong hydrogen bonds among wa
iVinArrow [24]
<span>A spider is small enough that its weight can be supported by surface tension, the relatively weak forces that hold water molecules together. </span>Water molecules "bond"<span> to other water molecules around them in all directions, so they tend to stick more strongly to their neighbors, forming a film-like layer across the surface</span>
7 0
3 years ago
A population of a wild animal is a frequent target for hunters. hunters tend to hunt the larger animals with big horns. how migh
DanielleElmas [232]
The smaller animals with short horns will be selected because the selection pressure, the hunters, favors their survival.
7 0
3 years ago
Read 2 more answers
Other questions:
  • For each cross, describe whether genetics or temperature determines the sex of progeny produced from eggs incubated at 35 degree
    8·1 answer
  • Indicate whether each description applies to a specific group of gymnosperms or to all gymnosperms. drag each item to the approp
    10·1 answer
  • State how the eye us adopted to its function
    13·1 answer
  • How do you determine the base sequence of a complementary DNA strand?
    7·1 answer
  • What is over utilisation?
    8·1 answer
  • A travel agency wants people to go on a tour of a mountain region with many glaciers. Write a paragraph for a travel brochure de
    11·2 answers
  • Identify the indentation that is inferiorolateral to the auricular surface. Identify the indentation that is inferiorolateral to
    10·1 answer
  • Guys please help me.......
    5·1 answer
  • Mike and Mack are identical twins. Which statement might explain why Mike developed lung disease and Mack did not?
    6·1 answer
  • White-tailed deer populations in the Texas Hill Country have been increasing for years. The area is now overpopulated
    9·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!