15. What's the requirement of every single natural process on Earth?
answer: Energy source
Mediastinal shift occurs in Tension pneumothorax. A tension pneumothorax makes the lung to collapse and the heart, the trachea and the great vessels, to shift toward the unaffected side of the chest. When air moves out from a laceration in the lung and goes in the pleural space or infiltrate the pleural space over a wound in the chest wall the shift happens.
Its a <span>polypeptide chain</span>
Answer:
The three main steps of PCR amplification are :
Denaturation
Annealing
Elongation
Explanation:
Polymerase Chain Reaction (PCR) can best be describes as a procedure to amplify or make copies of DNA fragments.
The basic steps involved for PCR are :
1. Denaturation
In this step, the two complementary strands of DNA are seperated by the usage of heat. The best temperature for this procedure is 95°C.
2. Annealing
After denaturation, each strand of DNA serves as a template foe building a new strand. During the process of annealing, the temperature is reduced so that the primers can attach to the DNA strands. The optimum for this depends on the nature of the primers but usually the most feasible temperature is 55°C.
3. Elongation
In this step, the DNA polymerase adds nucleotides to the primers attached to the templates. The optimum temperature For DNA polymerase to work is 72°C.
