Answer:
Yes The cap is present on all roots except those of parasites and in mycorrhizal roots .The roots of aquatic plants have vestigial caps when young but the initial soon die and the cap disappear. The calyptrogen initiate root cap in monocots. In the dicots the cap appears to be a specialized development of the epidermis. In Monocots the cap has attained independent origin.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Evaporation of the liquid
Hi,
The answer is A.
"The sensation stage of the erotic stimulus pathway theory is similar to the excitement and orgasm phases of the epor model."
Hope this helps!
r3t40
