Question

What is the texture and consistency of the dna? 2. why did we use a salt in the extraction solution? 3. is the dna soluble in the aqueous solution or alcohol? 4. what else might be in the ethanol/aqueous interface? how could you eliminate this? 5. which dna bases pair with each other? how many hydrogen bonds are shared by each pair?

Answer 1

Explanation:

These questions are related to the extraction of DNA using detergents and ethanol.

1. Consistency and texture- the extracted DNA is white, spongy and moist or slime in nature.

2. Salt is used during the extraction process as the DNA is negatively charged molecule and to neutralize the negative charge which will allow the formation of precipitate.

3. The DNA is soluble in aqueous solution as the DNA is a negatively charged molecule therefore the negative charge makes it a polar molecule and it can form the interactions in the aqueous solution.

4. RNA could be present in the aqueous solution along with the DNA as both are the nucleic acid.

5. The base-pairing rule was suggested by the Chargaff who proposed that Adenine binds thymine and guanine binds cytosine.

5. The binding of adenine to thymine involves two hydrogen bonds and between guanine to cytosine involves three hydrogen bonds.