The function of a vacuole is to store food or any other type of nutrients the cell might need to survive. This is true for both the plant and animal cell, though in the plant cell the vacuole's are much larger.
The correct answer is E.coli.
Eating undercooked meat puts you at a great risk of getting food-borne diseases, which are caused by pathogen microorganisms. One of the most common food-borne diseases from under-cooked beef is E.coli. The most common symptoms of E.coli are vomiting, diarrhoea and stomach cramps.