Answer:
e. None of the above
Explanation:
For me as a Researcher, the reason could be increased Concentration of your DNA sample which you are using as your template. Try to decrease the concentration of DNA (up to 100 ng per reaction is enough and can increase up to 200 ng). so the reason for getting non specific bands is increase concentration of DNA which results in non specific amplification and also degradation of DNA in the reaction which you can see in your gel electrophoresis results.
i always corrected my results using the same technique that is lowering the concentration of DNA between 100 and 200 ng per single reaction of PCR.
Answer:
D. GTP
Explanation:
Initiation of protein synthesis includes binding of mRNA to small ribosome subunit and initiation aminoacyl tRNA. The pairing of the anticodon of the initiating aminoacyl tRNA and initiation codon of mRNA requires GTP hydrolysis and marks the beginning of protein synthesis.
Elongation stage of protein synthesis requires movement of the ribosome along the mRNA which in turn is facilitated by GTP hydrolysis. As the ribosome moves along the mRNA, the codon of mRNA bind to the respective anticodon of aminoacyl tRNA by using the energy of GTP hydrolysis. The GTPase activity of elongation factor Ef-Tu ensures the fidelity of the process.
The release factors that recognize the stop codon and serve to mark the termination of protein synthesis use the energy of GTP hydrolysis during the process.
Answer:
Competition is an interaction between organisms or species in which both the organisms or species are harmed. Limited supply of at least one resource (such as food, water, and territory) used by both can be a factor.
Explanation:
Answer:land pollution (i think)
Explanation:
