Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
A
Explanation:
The possible number of amino acid residues in the peptide composed entirely of alpha-helical structure and found to contain an integer number of complete turns is 36
Answer:
33.5
Explanation:
the mean height is the average. Simply add up the height of all the plants with fertilizer and divide by 5 (Being the amount of plants with fertilizer)
30.5+32.1+40.6+30.5+33.8 = 167.5
167.5/5 = 33.5
AH(average height)= 33.5
It would be Kinetic and potential energy.
The answer to the above question is in a hot spring.
<h3>What is a habitat?</h3>
The term "habitat" in ecology refers to a region's collection of biotic, physical, and resource elements that are present to support a specific species' ability to survive and reproduce. It is possible to think of a species' habitat as the outward representation of its biological niche. As a result, "habitat" refers to a particular species, which is fundamentally distinct from ideas like "environment" or "vegetation assemblages," for which the term "habitat-type" is more applicable.
To learn more about habitat with the help of given link:
brainly.com/question/728057
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