The "scissors" of the molecular biology are: DNA Restriction enzymes.
A restriction enzyme is a protein capable of cleaving a DNA fragment at a characteristic nucleotide sequence called a restriction site. Each restriction enzyme thus recognizes a specific site. Several hundred restriction enzymes are currently known.
Naturally present in a large number of species of bacteria, these enzymes have become important tools in genetic engineering.
The "glue" of the molecular biology are: DNA ligase
In molecular biology, DNA ligases are ligase-class enzymes that catalyze the formation of a phosphodiester bond between two segments of DNA. DNA ligases are involved in several essential cellular processes of DNA metabolism: in DNA replication, suture of Okazaki fragments, and in DNA repair and homologous recombination.
The use of these tools in molecular biology: Cloning
Molecular cloning is one of the bases of genetic engineering. It consists of inserting a DNA fragment (called insert) in an appropriate vector such as a plasmid for example. The new plasmid thus created will then be introduced into a host cell, generally the Escherichia coli bacterium. This will then be selected and multiplied to obtain a large amount of the plasmid of interest. Cloning a gene involves inserting it into a plasmid. A clone will be the bacterial transformant that contains this particular plasmid. In this case we speak of clone because all the individuals of the bacterial colony are genetically identical. Molecular cloning is thus different from reproductive cloning (creating an individual genetically identical to another but of a different age) or therapeutic cloning (making tissues from stem cells to perform transplants compatible with the recipient).
Molecular cloning requires restriction enzymes capable of cleaving the DNA, and DNA ligase capable of re-gluing the DNA fragments. Ligase was isolated for the first time from T4 bacteriophage. This enzyme is involved in the repair and replication of DNA. It can bind DNA fragments with compatible sticky ends. At higher concentration, this enzyme is also able to bind two ends of DNA as shown here. T4 DNA ligase works using ATP and Mg ++. It has an activity optimum of 16 ° C, but remains active at room temperature.
Answer:
D) Each parent contributes one allele for this trait
Explanation:
All traits of individuals are determined by specific genes of that trait. For example, there is a certain gene for height, certain gene for eye color, face shape etc.
Genes are the units of hereditary, and for every trait there is one gene in every organism. However, one gene is present in two alternative forms called alleles in an organism. For example: There is a trait height, a person has two alleles for the height gene, one allele is for short height, and other allele is for tall height. The trait of tallness is dominant over the trait of shortness, Therefore, this person will have tall height.
Now the alleles are transmitted from parents to offspring. Every parent contributes one allele for a specific trait, in the process and transmit it to offspring.
The allele which will be dominant will be expressed while the one that is recessive will e suppressed.
Therefore, option D is the right answer.
Hope it helps!
Answer:
This sequence would target the peroxisome
Explanation:
- When the protein is made it will be able to target several organelles in the cell
- However, the C-terminal sequence is the last sequence transcribed and when the protein is complete this sequence will be the sequence used for targeting the specific organelle
- in this case, the C-terminal sequence targets the peroxisome
Answer:
Trees sprouting after a forest fire.
Explanation:
