Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
The answer is right below.
The bulk of this species’ diet consists of fish in the teleost infraclass, including dragonfish, rattails, anglerfish, and more. They are also known to feed on crustaceans and cephalopods like squid.
These sharks will prey on fish found both on the sea floor and in the water column. Because they are so slow moving, scientists believe these sharks are ambush predators. Their pinkish-red skin actually blends in when in the deep sea.
Answer:
B. The apple orchard releases toxic chemicals into the water of the bay.
Explanation:
Option B is the correct answer. It is the most likely explanation for the results in the graph.
From the graph, we can see that the population of the fishes sharply dropped. Since the fertilizer is applied to the apple orchard and the orchard was planted beside the bay, during the rain it is possible that the water has washed some of the fertilizers into the bay. The chemicals used in making fertilizer are harmful and toxic.
This reveals that the toxic chemicals released from the apple orchard are responsible for the decline in the population of the fish.
Answer
i dk the answer but u can do it i believe in you
Explanation:
A greater more than 3 because in a cube there are 6 numbers 1,2,3,4,5,6
Then it is not equal to have 1 greater than a tree has four numbers and less than three has 2. So more likely, Greater than three.
