Initially, Elise was concerned that the causative agent could have been Staphylococcus aureus, a Gram-positive bacterium, or P.
aeruginosa, a Gram-negative bacterium; both are common culprits in piercing infections. What culture methods would allow for isolation and differentiation of these two bacteria
One great differential method known is Gram staining. It uses certain dyes for the making of bacterial cells to standout over its background. The gram staining method is used in the differentiation of gram positive and gram negative organisms.
