Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Answer:
decomposers
Explanation:
These are heterotrophic organisms that feed on dead animals and plants, secretions, or discarded parts of living beings, that is, organic matter.
And they break it down into inorganic.
Thus, inorganic substances that can be reused in the process of photosynthesis are returned, recycled, to nature.
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➷ The correct answer would be D. Products of one process are used as some of the reactants for the other processes.
As you may know, the formula for photosynthesis is:
carbon dioxide + water ==> oxygen + glucose
And the formula for respiration is:
oxygen + glucose ==> carbon dioxide + water
The reactants for one are the products for the other and vice versa. This is why it would be the correct option.
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➶ Hope This Helps You!
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Hi! human,dog are multicellular organisms. Nucleus,cell membrane, cytoplasm and mitochondria are 4 things that plant and animal cells have.
They are all the examples of organ system.
