A 10-7 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, 0.1 mL of solution is

Question

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A 10-7 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, 0.1 mL of solution is

plated on solid media, and 66 colonies of bacteria grow on the plate. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture?

Biology

1 answer:

marishachu [46] · Answer 1 · 2022-09-03T17:42:35+03:00

Answer:

$66 * 10^8$ CFU/mL

Explanation:

Given -

a) Number of colonies of bacteria is equal to $66$

b) Total volume of the solution is equal to $0.1$ mL

c) Dilution factor is equal to $10^{-7}$

As we know that

CFU/mL $= \frac{N * DF}{V}$

Where N is the number of bacterial colonies, DF is the dilution factor and V is the volume

Substituting the given values in above equation, we get -

CFU/mL $= \frac{66* 10^7}{0.1} \\= 66*10^8$

A 10-7 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is

A 10-7 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, 0.1 mL of solution is