The answer is most likely D.
Answer:
Use a higher % agarose gel.
Explanation:
Agarose gels have a porous matrix. The higher the concentration of agarose, the smaller the pores, so larger DNA molecules will have more difficulty moving through the gel and they will run slower than small DNA molecules.
The higher % agarose gel has thus a better resolving power (the measurable interval between two entities -the DNA bands- is smaller). For that reason, a 2% agarose gel will allow you to differentiate better between two bands of close molecular weight, if you let the DNA fragments run long enough.
Answer:
d. DNA polymerase begins adding nucleotides at the 5' end of the template
Explanation:
Polymerase DNA always works in one direction, synthesizing the new 5' to 3' oriented chains and adding nucleotides to the 3' end of a new synthesis chain by forming phosphodiester bonds between the phosphate of a nucleotide and the sugar of the anterior nucleotide.
Because DNA polymerase only acts in a 5' to 3' direction, replication along a chain, the leading chain, occurs continuously. The synthesis of the opposite chain, the delayed chain, occurs discontinuously because the DNA polymerase must wait for the replication fork to open. Over the delayed chain, short segments of DNA called Okazaki fragments (named after Reiji and Tsuneko Okazaki, the scientists who discovered these fragments) are synthesized as polymerase DNA works out of the replication fork. Ligase DNA catalyzes the covalent bonds between Okazaki fragments in the delayed chain to ensure there are no gaps in the phosphodiester skeleton. Finally, the first ones are removed and these gaps are filled by the DNA polymerase.
Unlike exocrine glands, endocrine glands <span>release secretions directly into the blood. The correct option among all the options that are given in the question is the third option or option "C". Basically exo means outside and endo means inside. This is the hint that will lead to the correct answer.</span>
