Answer:
is bound to the constant region of the secondary antibody.
Explanation:
Enzyme immunoassays are the techniques used to detect the presence of antigens with the help of antibodies. Each of the antibody molecules has a constant and variable region.
The primary antibodies are added to the wells. The constant region of the secondary antibody is bound to an enzyme while its variable region is free so that it can bind to the specific antigen.
Addition of substrate to the system is followed by visualization and/or evaluation of antigen as the reaction between enzyme and substrate produce some visible changes such as color change.
It’s asking for the products of cellular respiration which are H2O,CO2
I am not too sure about this but
True
False
True
Well scientists will be able to study organisms more efficiently, and they will be able to organize them into groups based on their cell structure, behavior, habitat, similarity, and more.
Answer:
1. The difference between the normal hemoglobin protein DNA sequence and the sickle cell hemoglobin DNA sequence is a base to base shift, in this case adenine (GAG) to thymine (GTG).
2. The difference affects the amino acid sequence of the protein by replacing glutamic acid (Glu) with valine (Val).
Explanation:
In sickle cell anemia, a change in the DNA nucleotide sequence is observed, where adenine is substituted by thymine, whose expression is the change in the amino acid sequence of globine β, incorporating valine instead of glutamic acid. This represents a molecular mutation - point mutation - by subtitution, which corresponds to missense mutation.
<u>Normal hemoglobin protein in a RBC</u>
DNA CTG ACT CCT GAG GAG AAG TCT
Amino acids Leu Thr Pro Glu Glu Lys Ser
<u>Sickle cell hemoglobin protein in a RBC</u>
DNA CTG ACT CCT <em>GTG</em> GAG AAG TCT
Amino acids Leu Thr Pro <em>Val</em> Glu Lys Ser
When GAG is transcribed to mRNA, the CUC codon is obtained, which codes for glutamic acid. Thymine substitution causes the DNA sequence to change to GTG, which is transcribed as CAC, the codon that encodes the amino acid valine. The <u>change from glutamic acid to valine in β-globin causes an altered hemoglobin, giving the abnormal erythrocytes observed in sickle cell disease</u>.
