Answer:
DNA polymerases from human cells will denature under these conditions.
Explanation:
Under the PCR conditions described, DNA polymerase from <em>T. aquaticus </em>would be more effective than DNA polymerase from human cells. This is due to the higher optimal temperature of DNA polymerase from <em>T. aquaticus. </em>
Answer:
A. Glucose:
As lactose is absent will follow the glucose metabolism, so the lac repressor will hold tightly with the operator which leads to the prevention of transcription as RNA polymerase can not bind to promoter so no lactose metabolism.
B. lactose:
In the case or wild mutant lactose metabolism will occur as lactose is present, allolactose binds to the lac repressor thus the operator and promoter are free so transcription starts. The same result will be in all test except in the case of a mutant repressor.
C. glucose and lactose:
In this case, diauxic growth will occur. This growth takes place when two sugars are present in the media. The preferred sugar will be consumed first That is glucose. Then a lag phase will come which will be followed by the second sugar metabolism that is lactose. the given two cases :
Lactose will be metabolized first, as operator site mutation prevents repressor protein binding and thus will lead to the constitutive synthesis of lac operon in test B.
The same thing will happen, as in this case also, constitutive synthesis of lac operon will happen in test C.
Cultures grown in the presence of both glucose and lactose are metabolizing lactose. In the test (b) and (c) will be responsible due to constitutive synthesis of lac operon will happen.
Firstly, the enzyme, DNA Helicase breaks the Hydrogen bonds between a specific region of the DNA molecule and unwinds the duplex DNA molecule at the targeted gene sequence site.
Then, RNA Polymerase binds at the 3` site of the gene sequence of the sense/coding strand(only one strand) that acts as the template for mRNA synthesis. RNA Polymerase then matches free nucleotides by complementary base-pairing(A-U & C=G), working in the 5`to3` direction. Hydrogen bonds then form between the complementary bases, holding the nucleotides in place. Each free adjacent nucleotide is joined at 3` ends by Condensation reactions with the other to form Phosphodiester bonds.
Once the mRNA molecule is formed, it is separated from the synthesis complex and leaves the nucleus through pores in the nuclear envelope to moves towards ribosomes for Translation.
Lastly, the DNA double helix rewinds by forming Hydrogen Bonds.
The correct answer is option D, that is, it will increase the amount of carbon in the soil and increase the amount of carbon dioxide discharged by decomposers.
One of the ways to manage the garden is to utilize mulch, the mulch minimizes the contact between the soil and the organic matter so that the species will consume more time to dissociate it. The mulch will decompose and its nutrients will be discharged to the soil, however, it takes place very gradually, and enhances the organic matter in the soil.
If the mulch is left in the garden for a year, then the soil will be conserved and supplied with nutrients and this process will retain carbon in that garden. The sources of mulch can be leaves, grass, straw, or composted garden waste.
The nurses best response would be "Some jurisdictions have staffing laws which allows for nurses to be involved in staffing ratios".
