In oxidative phosphorylation that takes place in mitochondria, the uncouplers prevent the coupling in between the phosphorylation reactions and electron transport, and therefore, prevent the synthesis of ATP without influencing the ATP synthase and respiratory chain.
The uncoupler always exhibits its influence on the synthesis of ATP. The 2,4-dinitrophenol functions as an uncoupler at the time of oxidative phosphorylation. The NADPH and FAD functions as an electron carrier.
