Pancreatic cells, which secrete a large amount of digestive enzymes, are labeled with radioactive leucine and then chased for se
veral hours with nonradioactive leucine. Photographic emulsions are prepared at different times during the chase. Where would the black spots appear on an emulsion prepared 3 hours after the pulse?a. Secretory vesicles b. Rough ER
c. Exterior of the cell
d. Golgi apparatus
The pulse chase experiment was done to understand the secretion pathway of the protein at different time intervals. The protein travels through the endomembrane system for its secretion.
The cells of the pancreas are labelled with the leucine which is radioactive in nature. The black spots will be shown on the cell exterior as three hours are enough for the secretion of the protein outside the cell. This indicates that the protein has been secreted out of the cell.
If there wasn't a lot of organisms and energy in the productive part of the triangle, then there wouldn't be a lot of third level organisms. The energy piramid would be a lot smaller then what it is here, and maybe if the energy source was low then there might not be such a vast varity of animals in the ecosystem.
