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dolphi86 [110]
3 years ago
7

2.) If you wanted to express a large eukaryotic gene in a prokaryotic organism, what steps would be necessary? Describe your pro

tocol below including the suitability of a vector for a large gene.
Biology
1 answer:
alina1380 [7]3 years ago
5 0

Answer:

Explanation:

The eukaryotic genes of interest can be expressed in the prokaryotic genome or plasmid vector. The following are the steps used for the purpose of expression of eukaryotic genes in the prokaryotic genome.

1. Amplification of the target gene of interest from the eukaryotic genome by PCR or polymerase chain reaction.

2. The selection of the plasmid and digestion of the plasmid DNA with the restriction enzyme.

3. Ligation of the amplified DNA fragment and the plasmid.

4. Introduction of the ligated plasmid into the prokaryotic host organism.

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What type of muscle contracts and relaxes to control the movement of the contents?
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Smooth muscles are the type of muscle that contracts and relax to control the movement of the contents.

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2 years ago
In response to a drop in overall blood pressure, __________ stimulates constriction of the glomerular inlet and even greater con
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According to the research, angiotensin II (A) stimulates constriction of the glomerular inlet in response to a drop in overall blood pressure.

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3 0
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Dolphins, Tuna, and Jellyfish are known as the secondary consumers of sea life and prey on the primary consumers for a food source.

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Primary consumers such as Sea turtles, Manatees, and Zoo Plankton rely on the producers seaweed, plankton, and diatoms for food source. They are also known as the oceans herbivores.

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What is one defining feature of a prokaryotic cell?
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The distinguishing feature of a prokaryotic cell is that it has no nucleus or internal membranes, so unlike eukaryotic cells they contain no membrane bound organelles. - google
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