Answer:
The correct answer is e3
Explanation:
The given protein in the question proteib X contain four subunits of equal size alpha,beta,delta and gamma which are bound by either noncovalent interactions or disulfide bridges and only beta and delta subunits are bound to each other by disulfide bridges.
As a result when the protein will undergo fragmentation it will generate 3 bands because the interaction that is present between alpha and beta subunit will break resulting in the formation of 2 bands. Now the most important fact is that the beta subunit is linked to delta subunit by di sulfide bond so when betamarcaptoethanol will act on protein X it will break the disulsulfide bond present between the beta and delta subunit to reduce both the subunit.
Finally the interaction that is present between delta subunit and gamma subunit will be broken down to release the delta subunit which will form a single band during SDS PAGE..
Answer:
CHK2, p53, p21, cell cycle progression.
Explanation:
In a normal cell with no LFS mutation, during the G1 stage of cell cycle progression, the CHK2 activates if there is damage in the DNA. CHK2 activates p53, which is a tumor suppressor protein that will hold the cell cycle in G1/S until the DNA is repaired. The p53 protein activates p21, a protein that binds to CDK2 and stops the cell cycle. The cell cycle will continue once the damage is repaired.
In-text citations are for any information you got from another source. if you directly quote it, you need an in-text citation; if you summarize it, you need one; if you paraphrase it, you need one.
APA format in-text citations require three things if available: the author's name, the year, and the page number. for parenthetical citations, it's formatted like so: (Smith, 2002, pp. 91)
however, signal phrases change things. signal phrases are your lead-ins to quotes, like, "According to John Smith" or "An article by Jane Doe" -- HOWEVER, in APA format, you're required to put the year in the signal phrase if you mention the author. "According to John Smith" becomes "According to John Smith (2002)," and you continue your sentence like normal.
if no author is available, cite it by the title of the web article. for example: ("Camping," 2001). if no page number is available, as is usually the case with web sources, leave it out.
remember that if you say the article/page number/author's name IN your signal phrase, the same information doesn't need to be repeated in your in-text citation. you only need to give the information once, because they'll be able to find it on your reference page:
According to John Smith (2002), mosquitoes prefer humid weather (pp. 9).
Mosquitoes prefer humid weather (Smith, 2002, pp.9).
According to page 9 of "The Great Outdoors" by John Smith (2002), mosquitoes prefer humid weather.
note that for the last one, no "end of the sentence" in-text citation is needed, because you gave all three pieces of info within the sentence.
an easy way to remember the difference between MLA and APA is that APA emphasizes the date of your source more. it wants the year as soon as you reference your author. this is because in science fields, discoveries are often made then later revised, so they want to know what year you got your information from. outdated information could be considered like invalid or whatever so dates matter A Lot for science!!
MLA format is actually easier. all you have to do for the in-text citations is the author's last name and page number, or the article title. no dates are needed in-text.
as an example: (Smith 9)
no "pp." is needed as they require in APA format. just the name, then a number for the page. that's it. if you say the author's name in your signal phrase, you only need to give the page number:
According to John Smith in his book "The Great Outdoors," mosquitoes prefer humid weather (9).
According to "The Great Outdoors," mosquitoes prefer humid weather (Smith 9).
According to page 9 of "The Great Outdoors," mosquitoes prefer humid weather (Smith).
this...is long. but i hope it helps! feel free to message me if you run across any special cases that give you trouble.
