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irakobra [83]
3 years ago
6

The concentration of agarose gel affects the resolution of DNA separation. For a standard agarose gel electrophoresis, a 0.8% gi

ves good separation or resolution of large 5–10kb DNA fragments, while 2% gel gives good resolution for small 0.2-1kb fragments. 1% gels is often used for a standard electrophoresis. If you have very similar sized DNA molecules that are running too close together on an agarose gel, what solution would you apply to resolve this issue?
Biology
1 answer:
DIA [1.3K]3 years ago
7 0

Answer:

Use a higher % agarose gel.

Explanation:

Agarose gels have a porous matrix. The higher the concentration of agarose, the smaller the pores, so larger DNA molecules will have more difficulty moving through the gel and they will run slower than small DNA molecules.

The higher % agarose gel has thus a better resolving power (the measurable interval between two entities -the DNA bands- is smaller). For that reason, a 2% agarose gel will allow you to differentiate better between two bands of close molecular weight, if you let the DNA fragments run long enough.

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Answer:

D

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Pretty sure that's the correct answer.

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3 years ago
Your brain and neurons are in constant action, sending billions of ___________and________messages each day to keep everything, f
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Answer:

Until recently, most neuroscientists thought we were born with all the neurons we were ever going to have. As children we might produce some new neurons to help build the pathways - called neural circuits - that act as information highways between different areas of the brain. But scientists believed that once a neural circuit was in place, adding any new neurons would disrupt the flow of information and disable the brain’s communication system.

In 1962, scientist Joseph Altman challenged this belief when he saw evidence of neurogenesis (the birth of neurons) in a region of the adult rat brain called the hippocampus. He later reported that newborn neurons migrated from their birthplace in the hippocampus to other parts of the brain. In 1979, another scientist, Michael Kaplan, confirmed Altman’s findings in the rat brain, and in 1983 he found neural precursor cells in the forebrain of an adult monkey.

These discoveries about neurogenesis in the adult brain were surprising to other researchers who didn’t think they could be true in humans. But in the early 1980s, a scientist trying to understand how birds learn to sing suggested that neuroscientists look again at neurogenesis in the adult brain and begin to see how it might make sense. In a series of experiments, Fernando Nottebohm and his research team showed that the numbers of neurons in the forebrains of male canaries dramatically increased during the mating season. This was the same time in which the birds had to learn new songs to attract females.

Why did these bird brains add neurons at such a critical time in learning? Nottebohm believed it was because fresh neurons helped store new song patterns within the neural circuits of the forebrain, the area of the brain that controls complex behaviors. These new neurons made learning possible. If birds made new neurons to help them remember and learn, Nottebohm thought the brains of mammals might too.

Other scientists believed these findings could not apply to mammals, but Elizabeth Gould later found evidence of newborn neurons in a distinct area of the brain in monkeys, and Fred Gage and Peter Eriksson showed that the adult human brain produced new neurons in a similar area.

For some neuroscientists, neurogenesis in the adult brain is still an unproven theory. But others think the evidence offers intriguing possibilities about the role of adult-generated neurons in learning and memory.

if wrong report me

5 0
3 years ago
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Answer:

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Explanation:

Good luck!

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Answer:

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Explanation:

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