Researchers grew populations of identical Escherichia coli (E. coli) bacteria in a growth medium that contained a low concentrat
ion of glucose and a high concentration of citrate, a substance that is not typically consumed by E. coli. For thousands of generations, the bacteria used only glucose as an energy source and grew relatively slowly and to a low density because of the low concentration of glucose. After about 30,000 generations, one population emerged that began to rapidly grow to a much higher density. The researchers hypothesized that the bacteria evolved the ability to use citrate as an energy source and referred to them as Cit . To test the hypothesis, the researchers grew separate populations of the Cit bacteria and bacteria from the original population (Cit- ) in a growth medium that contained only citrate. Describe one outcome that would demonstrate that a given population has evolved.
The colony of bacteria is called cit positive, or cit in case these advance with colonization in media with citrate, since if they grow in number and proliferate, it means that they use citrate as a metabolite or food resource for their metabolism.
Explanation:
In case these are negative cit, the profileration would have to be null or see that the colony stagnant its growth, that is to say that they did not increase in number or even decrease their number layers and they did not replicate in media with citrate.
