The presence of a mutant lac repressor that could not bind lactose would result in no transcription even when lactose was present because the mutant repressor would remain bound to the lac operator.
Further Explanation:
When lactose is unavailable, the repressor molecule binds to the DNA and prevents transcription of the genes. Therefore, the enzyme that is required for the lactose metabolism will not be produced. Comparatively, in the presence of lactose, the repressor molecule will bind to inducer (allolactose), which prevents binding of the repressor to DNA results in transcription of a gene. Enzymes accountable for the lactose metabolism will be produced. The mutated repressor molecule, which prevents binding to DNA, will result in the production of enzymes all the time, irrespective of the presence or absence of lactose. Genes responsible for the metabolism of lactose will be expressed all the time. If a mutation occurs in the repressor, it will prevent inducer binding. The repressor molecule will bind then to DNA permanently and blocks the activity of RNA polymerase. This results in no transcription and translation of operon. Presence of a mutated operator that does not allow the binding of a repressor would cause constitutive expression of the operon.
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Answer Details:
Grade: High School
Subjects: Biology
Chapter: Genetics
Keywords:
Lactose, metabolism, genes, produce, DNA, allolactose, repressor, transcription, repressor, operon, binding, enzyme, absence.