The membrane bound organelle you refer to is the Lysosome. The lysosome fuses with a vacuole where it digest the contents.
Answer:
These facts are true and disturbing
Explanation:
Genetics is a discipline that has advanced incredibly in the last years. For example, by using the versatile CRISPR-Cas9 genome editing system, we are now able to insert literally any sequence into a given genome in vivo. The CRISPR-Cas9 is a technology that can be used in a regular molecular biology laboratory. It is expected this technique will enable in the near future to correct genetic disorders that have plagued mankind since times immemorial. However, genomic technologies like that could be used by malignant persons to hurt innocent people, thereby it is imperative that countries regulate their use.
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
The replication happens during the S phase.
