Researchers were trying to locate the Protein Clasp inside the cell. Immunofluorescence microscopy of STEK-1 cells was performed
to identify CLASP protein. Method - Gene fusion of clasp with GFP helped visualize Clasp in the cytosol. Now, they would like to study the interaction of Clasp with the cytoskeletal filaments, actin, and tubulin. They have the plan below. Will this work?
1 answer:
Answer:
*<em>This question includes a chart that helps to illustrate the subject and I'm attaching to the answer below...</em>
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Globular multi-functional proteins like actin and tubulin polymerize into chains of microfilaments working as cell skeletons, helping with the cells' motion and other processes such as cytokinesis which require these filaments to participate.
<em>Fluorescent Tagging</em> <em>(aka labeling)</em> helps in the detection of proteins and other biomolecules, this biotechnology method will help visualizing those specific actin and tubulin filaments, so yes, that will surely work.
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