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emmasim [6.3K]
3 years ago
6

how could you use genetic engineering techniques to make transformed bacteria that produce the enzyme

Biology
1 answer:
vovangra [49]3 years ago
8 0

Answer:

Genetic engineering is a process through which the genome of an organism is manipulated.

By recombinant DNA technology, we can manipulate the genome of bacteria and produce vital enzymes from it.

First of all, we select the genes for our desired protein or enzyme production. We cut the gene through specific restriction enzymes. The same restriction enzymes are used to cut a vector such as a plasmid. Then the gene of interest is placed into the plasmid. The plasmid or vactor is injected into a bacteria. As a result, the bacteria makes multiple copies of the gene and hence enzyme can be produced from it.

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46. The experiments that clearly showed that DNA from every organism studied had an amount of adenine equal to the amount of thy
Pepsi [2]

The given question number 48 is not complete, the complete question is:

Who utilized the process of transformation, as originally demonstrated by Frederick Griffith, to provide the first solid evidence suggesting that DNA was the genetic material of living systems (in this case bacteria)?

Messelson and Stahl

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Answer:

The correct answer of 46 is option d, that is, Erwin Chargaff.

The correct answer of 47 is option c, that is, G0 phase.

The correct answer of 48 is option e, that is, Avery, McCarty and Macleod.

Explanation:

46. Erwin Chargaff with the help of an experiment demonstrated that the amount of adenine present within the DNA is always equivalent to the amount of thymine present, similarly, guanine is always found in equal number to cytosine. He also demonstrated that pairing is always done between pyrimidines and purines within the DNA.  

47. It is in the G0 stage, also known as the quiescent stage, where a human cell is found in the non-proliferating phase. It is also considered as the resting stage of the cell cycle in which cell gathers enough of the substances for its nourishment. When the cell moves into the G0 stage of the cell cycle, then the cell either goes to further stages of the cycle when needed or goes through the process of differentiation.  

48. To prove that DNA works as a genetic substance, the Griffith experiment was further used by Avery, McCarthy, and Macloed. The experiment was again performed on a biochemical basis. In the experiment, distinct constituents of the bacterial cell were digested with the assistance of digestive enzymes, and in the process, it was found that when digestion of DNA was done, the process of transformation failed to take place. The performed experiment was also called the biochemical assay of transformation.  

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