The DNA replication products visualized during the sanger method of DNA sequencing are observed in which nucleotides are added.
Sanger sequencing is based on the process of DNA replication. A scientist creates a copy of his DNA strand. Then observe which nucleotides have been added. This way you can see the sequence of nucleotides. A laser excites the fluorescent labels in each band and a computer detects the resulting light.
Sanger sequencing produces extension products of various lengths ending in dideoxynucleotides at the 3' ends. Extension products are separated by capillary electrophoresis or CE. Molecules are injected by an electric current into a long glass capillary filled with gel polymer. Selective incorporation of chain-terminating dideoxynucleotides by DNA polymerases during in vitro DNA replication.
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Amoeba acquires it food by endocytosis, that is, a small region in the plasma membrane which ultimately forms a intra-cellular membrane-bound vescicle. It engulfs it food using its pseudopodia (temporary protrusion of the surface of the amoeboid cell used for feeding and movement) and forms a vacuole around it. When the food is already trapped inside, it releases a digestive enzyme to digest it. Intake of liquid particles is pinocytosis (cell drinking) while intake of solid particles is phagocytosis (cell eating).
A person that is homozygous<span> or </span><span>heterozygous</span>
Answer:
Measurement (Count) of Cell Numbers:
1. Breed Method:
A known volume of microbial cell suspension (0.01 ml) is spread uniformly over a glass slide covering a specific area (1 sq. cm). The smear is then fixed by heating, stained, examined under oil immersion lens, and the cells are counted.
Customarily, cells in a few microscopic fields are counted because it is not possible to scan the entire area of smear. The counting of total number of cells is determined by calculating the total number of microscopic fields per one square cm. area of the smear.
