Answer:
The correct answer will be-
1. Nucleosomes
2. Chromatin
3. Sister chroamtid
4. Centromere
Explanation:
The DNA is a very long molecule which if has to be passed on to the daughter cell as it is will cause problems. So, to reduce problems a cell form tightly packed structure of DNA so that they can be passed easily to the daughter cell.
The packaging of DNA begins with the wrapping of the DNA around histone proteins which forms the 11 nm basic structural units called nucleosomes. The nucleosomes start condensing each other and form 30 nm structure chromatin fibres.
The chromatin fibres undergo replication during S phase which produces an exact copy of the chromatin called sister chromatids bound to each other at a central point called centromere which helps the separation of the chromatids during M phase.
I'm sure it's true since before doing a science experiment/If you're trying to figure something out, you need a Hypothesis.
In human gene therapy, a genetically modified virus (a.k.a. a viral vector) can alter the genetic variation of a cell, but not all viral vectors do.
The process often begins with the delivery of or creation of a segment of viral double stranded DNA (containing the gene you want to introduce). Then typically an enzyme known as an integrase cuts the ends of the segment of viral DNA and also cuts open the cell's DNA. Then the viral DNA is integrated/ inserted into the cell's DNA. The connecting ends are ligated together and adjusted so that the nucleotide base pairs match up.
This in the future may affect the gene pool for instance if the viral DNA (your gene) was inserted in the middle of another gene or important regulatory sequence of the cell DNA, and this alteration may be passed on into offspring and become present in the gene pool, which could have bad effects.
The effects on the gene pool really depends on what the virus ends up doing. For example, it may fix the function of a damaged gene which is the goal, and allow for a working gene to be in the gene pool, which would be good. The problem with gene therapy is that it's difficult to predict 100% what the virus will do every time it is given to a patient.
But it's very important to consider that it will only affect the gene pool if the virus is able to enter and alter germ cells (reproductive cells). If the virus, enters somatic cells (regular body cells) this will not be passed on to future generations. So viruses can be designed to avoid germ cells and avoid this gene pool issue. Also, some viral vectors use viruses that do not integrate their DNA, the cells just express the viral DNA (create the desired protein from it) and over time the viral DNA is degraded/ lost which wouldn't pose this threat.
This is long, but I hope it helped!
