The thin layer of white blood cells and platelets found above the packed red cells in centrifuged blood is called the ______
1 answer:
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Answer and Explanation:
a. Why would such a heat-stable polymerase be beneficial in PCR?
- Because in PCR, DNA is heated up 95 °C to denature DNA (see first figure)
b. What would happen if it weren’t heat stable?
- If it weren't heat stable we had to add it in every PCR cycle and please note that PCR can take 20 to 35 cycles. Imagine being researcher that you need to open 20 small tubes every 5 minutes and add polymerase enzyme into these tubes for 20 to 35 times. It is very labor intensive and Taq polymerase relives the researchers from this work.
c. How might you choose a region of DNA for a PCR primer so as to increase the temperature necessary for primer annealing (to minimize nonspecific PCR products)?
- You need to calculate melting temperature (Tm) of your primers and use the calculated values to prevent non specific bindings. Primers usually binds non-specifically if the low annealing temperature is used (lower than 5 °C of your Tm value)
d. A PCR reaction begins with 5 double stranded segment of DNA. Estimate the number of double-stranded copies of DNA that are present after the completion of 15 amplification cycles?
In every amplification cycle copies of DNA are doubled. So the answer is 5 x 2^15.
The statement: Allele frequencies will stay the same, but genotype frequencies will change over time is False, option B
<h3>What are dominant and recessive traits?</h3>Dominant traits are traits which whenever the allele for such traits appear will always be expressed in the offspring.
Recessive traits are traits which are masked by the dominant trait whenever the allele for the dominant traits appear. Recessive traits are only expressed in the absence of the dominant traits.
In the snail population, the M is dominant over m and that selection acts against hom0zygous recessive individuals.
Also individuals in the population will only mate with others of the same genotype.
Overtime, it will be observed that the allele frequencies and the genotype frequencies will change over time.
In conclusion, the allele frequency and genotype frequencies of the snail population will change over time.
Learn more about allele and genotype frequencies at: brainly.com/question/6139888
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Note that the complete question is given below:
You are working with a population of snails. during the mating season, you observe that individuals in the population will only mate with others of the same genotype (for example, mm individuals will only mate with mm individuals). there are only two alleles at this locus (M is dominant; m is recessive); you have determined that the frequency of the M allele = 0.4 and that selection acts against h0m0zygous recessive individuals. Consider whatbwill occur in this population overbthe course of many generations and mark A for True or B for False for each of the following statements:
Allele frequencies will stay the same, but genotype frequencies will change over time.
