The answer should be 2 I'm pretty sure
Pasteurization kills microbes and prevents spoilage in beer, milk, and other goods. In his work with silkworms, Pasteur developed practices that are still used today for preventing disease in silkworm eggs. Using his germ theory of disease, he also developed vaccines for chicken cholera, anthrax, and rabies
Enlightenment = Meditating under Bodhi Tree.
Life of suffering = Life with religious ascetics.
Life given to pleasures = Life as Hindu prince.