You have already worked out that the white flower must have genotype pp (two recessive white alleles) and that the purple flower could be homozygous (PP) or heterozygous (Pp).
<span>Now you need to draw Punnet squares for both possibilities and show that the way to determine the genotype is the proportion of purple offspring. If the genotype is PP, then all the progeny will inherit one P and one p, making 100% purple flowers. If the purple flower is Pp, then half will inherit P and half will inherit p, making for half white and half purple flowers in the next generation.</span>
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The fragment and plasmid are both cut with the same restriction enzyme is the that help ensure that a new piece of DNA can be inserted inside a bacterial plasmid when the two are mixed together.
Explanation:
The gene cloning requires one of the necessary step of forming chimera in which gene or DNA sequence of interest in inserted into the plasmid of bacteria.
The gene of interest and plasmid is cut by using same endonuclease enzymes.
The endonuclease used are same because they produce fragments having same complementary ends which bond to form chimera or recombinant DNA.
The overhangs produced by endonuclease lets the gene and plasmid form base pair with each other.
If overhangs are not there DNA ligase performs the function of sealing the plasmid and gene.
